Association between methylation status of CpG island in DAT1 and DRD4 genes and clinical symptoms of ADHD
10.3969/j.issn.1002-0152.2017.02.007
- VernacularTitle:DAT1和DRD4基因启动子区甲基化与注意缺陷多动障碍临床症状的关联研究
- Author:
Shaohua WANG
;
Kaijing DING
;
Ruixiang LIU
;
Jie ZHANG
;
Runxu YANG
;
Huizhi ZHOU
;
Chen YANG
;
Lu LIU
;
Chuanyuan KANG
- Keywords:
Attention deficit hyperactivity disorder;
DNA methylation;
Dopamine transporter gene (DAT1/SLC6A3);
Dopamine receptor D4 gene (DRD4)
- From:
Chinese Journal of Nervous and Mental Diseases
2017;43(2):93-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of methylation status in DA T1 and DRD4 genes and severity of clinical manifestations in ADHD patients.Methods One hundrd eleven DSM-Ⅳ defined ADHD patients were enrolled in this study and the demographic data were collected.Clinical symptoms were also assessed by Attention Deficit Hyperactivity Disorder Rating Scale-Ⅳ Home Version (ADHD-RS-Ⅳ) and self-developed Oppositional Defiant Disorder (ODD) rating scale.Bisulfite genomic sequencing (BGS) was used to detect the methylation status of every CpG site in DA T1 and DRD4 promoter CpG island in peripheral venous blood.Results The DNA methylation level in total CpG island for DA T1 was higher in individuals without depression,anxiety or ADHD family history compared to individuals with above family histories (P＜0.05).The differences on methylation levels for DA T1 and DRD4 were not significant between high and low ADHD-RS-Ⅳ total score (≤30 vs.＞30),ADHD-RS-Ⅳ inattention score (≤ 17 vs.＞17),and ADHD-RS-Ⅳ hyperactivity/impulsivity score (≤13 vs.＞13) subgroups (all P＜0.05).The methylation levels in total CpG island in DA T1 was higher in individuals whose ODD score were ＜9 compared to those whose ODD score were ≥9 (P＜0.05).Conclusions Methylation status of CpG island in DAT1 may influence the severity of oppositional defiant symptom in ADHD patients,which is correlated with depression,anxiety and ADHD family histories.
