The study on Notch1 pathway-related tubular formation of glioma cell lines
10.3969/j.issn.1002-0152.2017.02.011
- VernacularTitle:Notch1通路与胶质瘤细胞管道形成能力相关性研究
- Author:
Qingping ZHANG
;
Xin MEI
;
Chao KE
;
Yinsheng CHEN
;
Furong CHEN
;
Jianliang CHEN
;
Zhongping CHEN
- Keywords:
Notch1;
Glioma;
3D cell culture;
Tubular formation
- From:
Chinese Journal of Nervous and Mental Diseases
2017;43(2):116-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the formation of glioma cells derived vessels (GCDV).Methods The tubular formation assay was performed on 3D cell cultures of U87,U251,U373,SF295,T98G,SKMG-4 and C6 glioma cell lines.The expression of Notch1 、Dll4 were examined by western blot analysis.Result The mean number of vasculogenic channels of cell lines C6,U373,U87,T98G,SF295,U251 and SKMG-4 per area (100×) were 25.2±5.0,36.4±3.2,19.0±2.2,12.6±2.4,4±2.2,0.2±0.4 and 0,respectively.The relative protein densities of Notchl in U87,U251,T98G,SF295,SKMG-4,C6 and U373 were 0.34,0.21,0.79,0.04,0.28,1.75 and 1.19,which were significantly related with the tubular formation ability (P＜0.05).However,the expression of Dll4 was not associated with tubular formation ability (P＞0.05).Conclusion Notch1 may play a key role in tubular formation of glioma cells while the role of Dll4 in the process needs further study.
