Simultaneous determination of four saikosaponins in Xiaozheng Pellets by QAMS
10.3969/j.issn.1001-1528.2017.08.016
- VernacularTitle:一测多评法同时测定消癥微丸中4种柴胡皂苷
- Author:
Yanming XIA
;
Ying DONG
;
Liuqing DI
;
Xiaoli ZHAO
- Keywords:
Xiaozheng Pellets;
saikosaponin a;
saikosaponin b1;
saikosaponin b2;
saikosaponin c;
QAMS
- From:
Chinese Traditional Patent Medicine
2017;39(8):1620-1625
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of four saikosaponins in Xiaozheng Pellets (Bupleuri Radix,Cyperi Rhi-zoma,Rhei Radix et Rhizoma,etc.).METHODS The analysis of 5％ ammonia-methanol extract of this drug was performed on a Waters Xbridge C18column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210 nm and 254 nm.With saikosaponin a as a internal standard,the relative correction factors of saikosaponins b1,b2 and c were calculated,followed by the determination of their contents.RESULTS Four saikosaponins showed good linear relationships within their own ranges (r ≥ 0.999 5),whose average recoveries were 98.08％-102.94％ with the RSDs of 0.85％-1.82％.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,precise and feasible method can be used for the quality control of Xiaozheng Pellets.
