Drug resistant mechanism and homology analysis of carbapenem-resistant Klebsiella pneumoniae isolated from different sites of one patient
10.13602/j.cnki.jcls.2017.07.08
- VernacularTitle:同一患者不同部位分离耐碳青霉烯类肺炎克雷伯菌耐药机制研究及同源性分析
- Author:
Yongmei HU
;
Mingxiang ZOU
;
Jun LI
;
Qingya DOU
;
Haichen WANG
;
Qun YAN
;
Wenen LIU
- Keywords:
multiple-site infection;
Klebsiella pneumoniae;
New Delhi metallo-β-lactamase-1;
multilocus sequence typing
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(7):511-513,517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the drug resistant mechanism and homology of three strains of carbapenem-resistant Klebsiella pneumoniae (K.pneumoniae) isolated from different sites of one patient.Methods Three strains of carbapenem-resistant K.pneumoniae were isolated from femoral vein catheter tip,wound secretions and sputum of a patient with severe burns,respectively.Their carbapenemase,metallo-β-lactamase (MBL) and drug resistance genes were detected by modified Hodge test,double-disk synergy test and combination disk diffusion and PCR,respectively,and homology and biological typing were analyzed by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) assay and multilocus sequence typing (MLST) technology,respectively.Results The carbapenemase and MBL of three strains of carbapenem-resistant K.pneumoniae were negative and positive,respectively.The blaNDM-1 gene was identified from the three strains,but other drug resistance genes such as blanC,blaGES,blaIMP,blaSPM,blaVIM,blaGIM and blaOXA-48 were not detected.ERIC-PCR showed that three isolates belonged to the same genotype,and MLST showed that they were type ST17.Conclusion Carring blaNDM-1 gene is the main cause leading to the drug resistance of three strains of carbapenem-resistant K.pneumoniae,and they belong to the same genotype.
