Evolution analysis and trend prediction of neuraminidase gene of influenza A/H1N1(09pdm) virus in China from 2009 to 2016
10.13602/j.cnki.jcls.2017.07.19
- VernacularTitle:2009至2016年中国甲型H1N1流感病毒神经氨酸酶基因进化分析及流行趋势预测
- Author:
Yue SONG
;
Wenjuan YAN
;
Kun FANG
;
Xiaoshan LI
;
Wei LI
;
Pingmin WEI
- Keywords:
influenza A/H1 N1 (09pdm);
neuraminidase;
molecular characteristics;
evolution analysis
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(7):550-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the evolutionary characteristics and predict the variation trend of neuraminidase (NA) gene of influenza A/H1N1 (09pdm) virus in China from 2009 to 2016 in order to provide the basis of assessment for flu vaccines.Methods A total of 1 141 sequences of NA gene of influenza A/H1N1 (09pdm) virus were screened out from the Global Initiative on Sharing All Influenza Data and National Center for Biotechnology Information.The phylogenetic trees and the mutations of amino acids sequences were constructed and analyzed by biological softwares.The prediction for epidemic trend of influenza was analyzed by Bayesian skyline Plot.Results Compared with the sequence of reference strain,the homology of nucleic acid sequence of NA gene decreased year by year from 2009 to 2016.The phylogenetic analysis showed that NA gene clustered nearly on the identical phylogenetic tree in one year.The positive selection pressure site of NA strain was observed by different models in each year except 2012.The dynamics analysis showed that the popularity of influenza A/H1N1 virus may continue to increase to a peak in 2017.Conclusion The amino acid encoded by NA gene of influenza A/H1N1 virus is varying gradually,so the importance of surveillance for influenza virus should be reinforced for every year.
