Effect of Supplemented Buyang Huanwu Decoction on blood glucose in diabetic rats and its antioxidant activity
10.3969/j.issn.1001-1528.2017.08.003
- VernacularTitle:加味补阳还五汤对糖尿病大鼠血糖的影响及其抗氧化活性
- Author:
Jian MOU
;
Guihua ZHUANG
;
Hongli SHANG
- Keywords:
Supplemented Buyang Huanwu Decoction;
blood glucose;
antioxidant activity;
calycosin-7-O-β-D-glucoside;
peoniflorin;
diabetic rats
- From:
Chinese Traditional Patent Medicine
2017;39(8):1561-1566
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of Supplemented Buyang Huanwu Decoction (Astragali Radix,Angelica tail,Paeoniae Radix rubra,etc.) on blood glucose in diabetic rats and its antioxidant activity.METHODS The diabetic rat model induced by streptozotocin (STZ) was established,with metformin as positive control group.After intragastric administration with Supplemented Buyang Huanwu Decoction,the fasting blood glucose,superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum and tissues (heart,kidney and pancreas) in rats were detected.HPLC was used to determine the contents of antioxidant constituents (calycosin-7-O-β-D-glucoside and peoniflorin) in plasma,whose pharmacokinetic parameters were then calculated.RESULTS Compared with the model group,the hypoglycemic activity in the Supplemented Buyang Huanwu Decoction group was obvious (P ＜ 0.05),the SOD activity in serum and various issues (except for pancreas) was significantly enhanced,together with significantly reduced MDA level (P ＜ 0.05).The pharmacokinetic behavior of two constituents (calycosin-7-O-3-D-glucoside and peoniflorin) accorded with two-compartment open model,whose blood concentrations reached the highest within 50-70 min,and showed no obvious changes within 180-720 min.CONCLUSION Supplemented Buyang Huanwu Decoction can reduce the blood glucose in diabetic rats and improve the antioxidant activities in heart and kidney.The fast absorption and slow metabolism of calycosin-7-O-3-D-glucoside and peoniflorin in decoction are beneficial to related treatment.
