Simultaneous determination of fourteen constituents in Yangxue Qingnao Granules by LC-MS/MS
10.3969/j.issn.1001-1528.2017.08.015
- VernacularTitle:LC-MS/MS法同时测定养血清脑颗粒中14种成分
- Author:
Yaqing GUO
;
Xiaoming WANG
;
Guixiang PAN
;
Tong YIN
;
Wenfang LI
;
Haohao CHEN
- Keywords:
Yangxue Qingnao Granules;
chemical constituents;
LC-MS/MS
- From:
Chinese Traditional Patent Medicine
2017;39(8):1616-1620
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an LC-MS/MS method for the simultaneous content determination of paeoniflorin,albiflorin,neochlorogenic acid,chlororogenic acid,catechin,epicatechin,ethyl gallate,danshensu,ferulic acid,caffeic acid,gallic acid,protocatechuic acid,protocatechualdehyde and rosmarinic acid in Yangxue Qingnao Granules (Angelicae sinensis Radix,Chuanxiong Rhizoma,Paeoniae Radix Alba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 20 ℃ thermostatic Waters Symmetry Shield RP C18 column (3.9 mm × 150 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.1％ formic acid) flowing at 0.4 mL/min in a gradient elution manner.RESULTS Fourteen constituents showed good linear relationships within their own ranges,whose average recoveries were 85.5％-107.0％ with the RSDs of 2.0％-5.0％.CONCLUSION This simple,sensitive,accurate and reproducible method can be used for the quality control of Yangxue Qingnao Granules.
