Simultaneous determination of four constituents in Compound Kendir Leaves Tablets Ⅰ by HPLC-DAD
10.3969/j.issn.1001-1528.2017.08.017
- VernacularTitle:HPLC-DAD法同时测定复方罗布麻片Ⅰ中4种成分
- Author:
Weikui WU
;
Qianru YAN
;
Zhenyu GUO
;
Junxuan YANG
- Keywords:
Compound Kendir Leaves Tablets Ⅰ;
chlorogenic acid;
buddleodide;
hydrochlorothiazide;
promethazine hydrochloride;
HPLC-DAD
- From:
Chinese Traditional Patent Medicine
2017;39(8):1625-1628
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-DAD method for the simultaneous content determination of four constituents in Compound Kendir Leaves Tablets Ⅰ (Apocyni veneti Folium,Chrysanthemi indici Flos,Stephaniae tetrandrae Radix,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Shimadzu VP-ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of methanol-0.5％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 260 nm and 325 nm.RESULTS Chlorogenic acid,hydrochlorothiazide,buddleodide and promethazine hydrochloride showed good linear relationships within the ranges of 24.91-498.2 ng (r =0.999 9),286.33-5 726.7 ng (r =0.999 9),10.04-200.9 ng (r =0.999 9) and 154.80-3 096.1 ng (r =0.999 9),whose average recoveries were 98.3％ (RSD =1.3％),99.1％ (RSD =0.6％),98.5％ (RSD =1.0％) and 99.3％ (RSD =1.2％),respectively.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Compound Kendir Leaves Tablets Ⅰ.
