The effects of low-frequency electroacupuncture on hyperalgesia PKA-TRPV1 signal pathway and neurotransmitters in the spinal cord of rats with neuropathic pain induced by spared nerve injury
10.3760/cma.j.issn.0254-1424.2017.07.001
- VernacularTitle:低频电针对选择性神经损伤大鼠神经痛维持期脊髓背角PKA-TRPV1通路及痛敏递质的干预作用
- Author:
Jiayu YE
;
Yongliang JIANG
;
Sisi YAN
;
Xiaofen HE
;
Junying DU
;
Wensheng ZHAO
;
Xiaojun CHEN
;
Lifang CHEN
;
Jianqiao FANG
- Keywords:
Spared nerve injury;
Neuropathic pain;
Electroacupuncture;
Transient receptor potential V1;
Protein kinase A;
Spinal cord
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2017;39(7):481-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of low-frequency electroacupuncture (EA) on neuropathic pain induced by spinal nerve injury and its underlying mechanism.Methods Thirty-two male Sprague-Dawley rats were randomly divided into a normal group,a sham spared nerve injury (SNI) group,an SNI group and an SNI+EA group,each of 8.The rats in the SNI and SNI+EA groups were given SNI surgery,while those of the sham-SNI group only had the sciatic nerve and its branches exposed without any lesion.EA at 2 Hz was applied over the ipsilateral Zusanli and Kunlun acupoints daily for 14 days after the surgery.The ipsilateral paw withdrawal threshold (PWT) was measured,along with protein kinase A (PKA) levels in the dorsal horn of the spinal cord,calcitonin gene-related peptide (CGRP) and substance P (SP) levels along with transient receptor potential V1 (TRPV1).Results Compared to the normal group,the SNI groups all showed significant decreases in their PWTs on the affected side and significant increases in PKA,TRPV1,CGRP and substance P on the affected side.Compared to SNI group,the average ipsilateral PWT in the SNI+EA group increased significantly after EA treatment,while PKA levels,TRPV1,CGRP levels and SP expression all decreased significantly.Conclusion Electroacupuncture at low frequency can effectively relieve neuropathic pain,perhaps through down-regulation of PKA in the spinal cord and by decreasing pain hypersensitivity related to CGRP and SP.
