Study on Mathematical Model and Simulation of Competition-type Lateral Flow Immunoassay
10.11895/j.issn.0253-3820.170281
- VernacularTitle:竞争法免疫层析试条的数学模型与仿真研究
- Author:
Nianyin ZENG
;
Panpan ZHU
;
Yurong LI
;
Haiyan JIANG
;
Min DU
- Keywords:
Lateral flow immunoassay;
Competition-type;
Convection-diffusion equation;
Mathematical model;
Biochemical reaction process
- From:
Chinese Journal of Analytical Chemistry
2017;45(9):1284-1290
- CountryChina
- Language:Chinese
-
Abstract:
A mathematical model of competition-type lateral flow immunoassay (LFIA) was developed to describe the dynamic process of LFIA.The competition-type LFIA was divided into two categories: TwA-competition-type LFIA and TnA-competition-type LFIA.On the basis of the developed model, the COMSOL software was exploited to simulate the dynamic process of LFIA.The simulation result demonstrated the relationships between the concentrations of substances on the test and control lines and the influence factors.In particular, the influence factors in the TwA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the position of the test line (5-20 mm).On the other hand, the influence factors in the TnA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the porosity.Experiment result showed that the developed model could be used to explore the influence of the parameters on the test results, and optimize the performance of LFIA.
