Study on the absorption of monosialotetrahexosylganglioside sodium in rats with in situ cycle intestinal perfusion methods

VernacularTitle:单唾液酸四己糖神经节苷脂钠大鼠在体循环肠灌流吸收探究
Author: Long DENG ; Yao WANG ; Meiling CHEN ; Ying XIONG ; Jiaqi LUO ; Shufeng TAN ; Wei WU ; Wen ZHOU ; Dejian JIANG ; Xiaoqing WANG
Keywords: G(M1) ganglioside/PK; Intestinal absorption; Perfusion
From: Journal of Chinese Physician 2017;19(4):510-513
CountryChina
Language:Chinese
Abstract: Objective To investigate the duodenum absorptive character of monosialotetrahexosylganglioside sodium (GM1) in rats.Methods The contents of phenolsulfonphthalein (as indicators) and GM1 were determined with ultraviolet-visible (UV) method and high performance liquid chromatography (HPLC) method in rats with in situ cycle intestinal perfusion model.Results The ratio of duodenum absorption of GM1 was 10％ in 2 h after cycle and 22％ in 6 h after cycle,respectively.The Ka was (0.030± 0.012)h,and absorption t1/2 was (25.50 ± 8.56)h in 8 h after cycle.Conclusions GM1 is absorption in rat duodenum,and the accumulate absorption of GM1 is almost linearly related to the cycle time.The absorption dynamics of GM1 may be first-order kinetic process.