Comparison of Reversed Phase High Performance Liquid Chromatography and Fluorescence Polarization Immunoassay for the Determination of Plasma Concentration of Carbamazepine
10.3870/j.issn.1004-0781.2017.09.024
- VernacularTitle:反相高效液相色谱法与荧光偏振免疫法测定血浆卡马西平浓度比较
- Author:
Wanjun BAI
;
Xiaoli SUN
;
Jing AN
;
Haojing SONG
;
Zhanjun DONG
- Keywords:
Carbamazepine;
Chromatography,high performance liquid,reversed phase;
Fluorescence polarization immunoassay;
Drug concentration in plasma
- From:
Herald of Medicine
2017;36(9):1043-1046
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the correlation and difference of reversed phase high performance liquid chromatography (RP-HPLC) and fluorescence polarization immunoassay (FPIA) on determining serum concentration of carbamazepine.Methods Fifty serum samples were collected,both RP-HPLC and FPIA methods were employed to determine the concentration of carbamazepine.The results were analyzed by paired t test,Bland-Altman and Deming regression methods,respectively.Results The results of measuring 50 samples by the two methods showed that FPIA datas were significantly higher than RP-HPLC datas,and there was statistically significant difference(P<0.05) and poorer consistency between two methods;There was good correlation between carbamazepine concentrations determined by the two methods.Deming regression equation was CFPIA=1.195 3 CRP-HPLC-0.144 0,and Pearson correlation coefficient was 0.968 5.Conclusion Clinicians should pay more attention to the difference of carbamazepine concentration determination by different methods when carbamazepine individualized dosage regimen was adjusted according to therapeutic drug monitoring.
