Detection of minimal residual disease in children with acute lymphoblastic leukemia and its application

VernacularTitle:儿童急性淋巴细胞白血病微小残留病的检测及其应用进展
Author: Xiang QIN ; Wenjun LIU
Keywords: Acute lymphoblastic leukemia; Minimal residual disease; Flow cytometry; Quantitative real-time polymerase chain reaction; Immune repertoire sequencing
From: Chinese Journal of Applied Clinical Pediatrics 2017;32(15):1198-1200
CountryChina
Language:Chinese
Abstract: Childhood acute lymphoblastic leukemia (ALL) is a kind of abnormal proliferation of malignant tumor diseases originated in lymphocytes,which is the most common malignant tumor in children.Although stratified treatment has significantly improved the efficacy of ALL in children,but 15％-20％ of the patients still have ultimately relapsed due to the minimal residual disease(MRD).MRD refers to the leukemia patients after induction chemotherapy complete remission (or bone marrow transplant),in vivo residual morphology which could not be detected in trace amounts of leukemia cells.The detection methods of MRD mainly include flow cytometry,quantitative real-time polymerase chain reaction and immune repertoire sequencing.MRD level detection is of great important to judge the prognosis,and to guide the risk grouping and individual treatment,and so on.Now,the progress in the clinical application of MRD in children with ALL in recent years was reviewed.