Overexpression of IL-18 gene by T lymphocytesenhances their cytotoxic effect against pancreatic cancer cells SW-1990
- VernacularTitle:过表达IL-18的T细胞对胰腺癌细胞SW-1990的杀伤作用
- Author:
Genshen ZHONG
;
Duo YOU
;
Yanan CHEN
;
Ru YANG
;
Zhishan XU
;
Minna WU
- Keywords:
pancreatic cancer;
IL-18;
T cell
- From:
Basic & Clinical Medicine
2017;37(9):1251-1256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor effects of IL-18 gene transfected T cells on pancreatic cancer cell SW-1990.Methods Construction of IL-18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the human T cells and evaluated the antitumor effects when cocultured with SW-1990.Results The IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and transfected the T cells, the LDH secretion and IL-2 and IFN-γ content all increased significantly (P<0.01) when cocultured with SW-1990 cell.Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer cell SW-1990 as compared to the regular T cells.
