Determination of Four Saponin Components in Paridis Rhizhma by Quantitative Analysis of Multi-components with Single-marker
10.3870/j.issn.1004-0781.2017.09.020
- VernacularTitle:一测多评法测定重楼药材中4种皂苷类成分含量
- Author:
Saihua XU
;
Yuxiang SHEN
- Keywords:
Paridis rhizhma;
Quantitative analysis of multi-components with single-marker;
Correlation factors,relative
- From:
Herald of Medicine
2017;36(9):1029-1033
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality evaluation method for determination of paris saponinⅠ,Ⅱ,Ⅵ,Ⅶ in paridis rhizhma by quantitative analysis of multi-components with single-marker (QAMS).Methods An HPLC method and a Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm)were used.The mobile phase was acetonitrile-water (48:52) at a flow rate of 1.0 mL·min-1.The detection wavelength was 203 nm and column temperature was 25 ℃.Parissaponin Ⅶ was used as the internal reference substance.The relative correlation factors of parissaponinⅠ,Ⅱ,Ⅵ were calculated by standard curve method and QAMS.Results The QAMS method could be used for determination of four saponin components at the same time without significant difference as compared with the results of standard curve method (RSD<2.0%).Conclusion QAMS method is simple and reproducible,which can provide a reference for quality standard revision for paridis rhizhma.
