Effects of Guttiferone K on Inducing G0/1 Arrest of Prostate Cancer LNCaP Cells

VernacularTitle:Guttiferone K诱导人前列腺癌LNCaP细胞Go/1期阻滞的作用研究
Author: Wei ZHAI ; Jiling FENG ; Zhichao XI ; Hongsheng TAN
Keywords: Guttiferone K; cell cycle; prostate cancer; anti-cancer effect
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(2):235-240
CountryChina
Language:Chinese
Abstract: This study aimed at investigating the effects of Guttiferone K (GUTK),a polycyclic polyprenylated acylphloroglucinols (PPAPs) compound isolated from Garcinia yunnanensis,on the cell proliferation of human prostate cancer LNCaP cell line.Human prostate cancer LNCaP cells in the period of logarithmic phase were treated with GUTK.MTI assay was used to detect cell proliferation.Flow cytometry of propidium iodide (PI) staining,BrdU assay and immunocytochemistry were adopted to analyze the cell cycle phase distribution.Protein levels of Cyclin A,p27 and SKP2 were detected by western blotting.The results showed that GUTK inhibited the proliferation and induced G0/1 arrest of LNCaP cells in a time and dose dependent manner.The protein levels of Cyclin A and SKP2 were decreased,while p27 was increased by GUTK in human prostate cancer LNCaP cells.It was concluded that GUTK,a compound isolated from G.yunnanensis,presented the effect of inhibiting the cell proliferation by inducing G0/1 arrest of human prostate cancer LNCaP cells,with potential anti-prostate cancer action.