A Pharmacodynamic Study on the Anti-Herpes Simplex Virus (HSV) Activity of the Polysaccharides and Gels from Prunella vulgaris L.
10.11842/wst.2017.02.010
- VernacularTitle:夏枯草多糖及凝胶抗单纯疱疹病毒的药效学研究
- Author:
Shuangfan CAI
;
Yang YANG
;
Rong WU
;
Yifu YANG
;
Hongsheng TAN
- Keywords:
Prunella vulgaris L.;
anti-herpes simplex virus;
polysaccharides;
gel
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(2):247-253
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to explore the anti-HSV activity of P.vulgaris polysaccharides and gels in vitro and in vivo with the provision of scientific evidence for the further research of anti-HSV new drugs.Plaque reduction assay was adopted to determine the IC50 value of P.vulgaris polysaccharides on HSV-1 and HSV-2 in vitro.In addition,HSV-1 (skin) and HSV-2 (vulva) infected guinea-pig models were established for assessing the anti-HSV activity of polysaccharides and gels in vivo,and infected lesion degree,number of papulovesicle,typical lesion score and the DNA copy numbers of HSV-1 and HSV-2 viruses in the lesion tissue were taken as the indexes.It was found that the activities of HSV1 and HSV-2 viruses was inhibited by P.vulgaris polysaccharides in vitro,while HSV induced skin lesions in the guineapigs were ameliorated by P.vulgaris gel,exerting an anti-HSV action.In conclusion,it was demonstrated that P.vulgaris polysaccharides and gels performed an anti-HSV action both in vitro and in vivo with the hidden value of developing antiHSV agents.
