The Establishment of Autoimmune Premature Ovarian Failure (POF) Model Using B6AF1 Female Mice
10.11842/wst.2017.02.021
- VernacularTitle:B6AF1雌性小鼠免疫性卵巢早衰模型建立
- Author:
Peijuan WANG
;
Si CHEN
;
Yan LU
- Keywords:
Premature ovarian failure;
B6AF1 female mice;
mouse model;
immunology
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(2):319-324
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at exploring the modeling and identification method of autoimmune POF by the ZP3 polypeptide with the provision of scientific basis for the prevention and treatment of POF.B6AF1 female mice,the hybridization of A/J male mice and C57BL/6 female mice,was immunized by zona pellucida polypeptide fragments.Then,the mice estrous cycle was detected by vaginal exfoliated cells Pasteur's staining.After the 14-day modeling,the mice model was identified on the 7th and 14th day,respectively.Ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change were detected by H&E staining and ZP3 immunofluorescence staining.Serum hormone levels of E2 and FSH in the mice were tested by ELISA,with the combination of estrous cycle,ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change to judge the success of the POF mice model.It was found that ZP3 polypeptide immunized mice appeared the disorder of estrous cycle after 14 days,so did the marked inflammation observed by ovarian biopsy,a clear transparent zone by ZP3 immunofluorescence staining,and the decrease of serum E2 declined and the increase of serum FSH by ELISA.However,no significant change was found on the 7th day in the modeling.In conclusion,ZP3 polypeptide induced autoimmune POF mice model was established successfully,featuring high incidence and simple method and laying a foundation for the further study of autoimmune POF.
