The Determination of Major Effective Compounds in Gardeniae Fructus from Different Regions and the Establishment of Fingerprints Based on UPLC
10.11842/wst.2017.02.025
- VernacularTitle:基于UPLC技术测定栀子主要有效成分及特征指纹图谱研究
- Author:
Zhaoxing LI
;
Jie SHEN
;
Chunnian HE
;
Xuegang LI
;
Yong PENG
- Keywords:
Gardenia Fructus;
ultra-performance liquid chromatography;
fingerprint;
iridoids;
crocins;
quantitative analysis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(2):344-350
- CountryChina
- Language:Chinese
-
Abstract:
For promoting the quality evaluation of Gardenia resource,a UPLC method for the determination of 4 compounds (geniposidic acid,geniposide,crocin-1 and crocin-2) in Gardeniae Fructus was established.The UPLC separation was performed on an Waters Acquity UPLC BEH-C18 (2.1 mm× 100 mm,1.7 μm) column eluted with the mobile phases of acetonitrile-0.1％ formic acid solution in a gradient mode at a flow rate of 0.3 mL· min-1.The detection wavelengths were 240 nm and 440 nm,respectively.Under the optimum conditions,the calibration curves of four analysis were linear in the range of 0.0065-0.0096 μg· mL-1,with correlation coefficients more than 0.9995.The limits of detection (LODs) of four analysis were in the range of 0.0386-0.1875 μg· mL-1.As the results of determination of 4 compounds of 12 batches of Gardeniae Fructus showed,there was great differences between the contents of the 4 compounds,the contents of geniposide were 2.44％-6.96％,while the contents of crocin-1 were 1.26％-3.04％.In addition,fingerprints of 12 batches of Gardenia resources were built using ChemPattern software,and analysis and exploration over the detection results of several samples were carried out using multivariate statistical method (similarity analysis,principal component analysis and cluster analysis).The present study provided a reference to value the importance of this method in the quality evaluation and quality control of Gardeniae resources and slices.
