Isolation and Adherent Culture of Neural Stem Cells from Mouse Embryo
10.3870/j.issn.1672-0741.2017.03.015
- VernacularTitle:小鼠胚胎神经干细胞的分离及贴壁培养方法
- Author:
Qichang YANG
;
Xiaofeng SONG
;
Ping HAN
- Keywords:
neural stem cells;
adherent culture;
coating process;
immunofluorescence;
suspension culture
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2017;46(3):312-316,340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a modified method of adherent culturing NSCs to replace the traditional suspension culturing method.Methods Neural stem cells(NSCs)were isolated from brain of fetal mouse at 15.5-day fetal age.In culture bottle which had been pre-coated with poly-L-ornithine hydrochloride/fibronectin(PO/FN),the cells were adherently cultured and passaged.The cell morphology was observed under inverted microscope.NSCs and their differentiated cells were identified by immunofluorescence.Results NSCs obtained in this study were successfully adherently cultured and expressed specific markers.Conclusion NSCs are successfully adherently cultured in this study.As compared with the traditional suspension culturing method,adherent culturing is simpler and has lower wastage of passage.It is easy to observe cell morphology and differentiation by this method.It is also helpful to culturing and storage of NSCs.
