Protective Effects of Zhen-Gan Xi-Feng Decoction-containing Serum on 6-OHDA-induced Oxidative Stress in PC12 Cells through Nrf2-ARE Pathway Activation
10.11842/wst.2017.03.017
- VernacularTitle:镇肝熄风汤含药血清激活Nrf2/ARE信号通路对6-OHDA诱导PC12细胞氧化应激的保护作用
- Author:
Xuemei ZHAO
;
Tianjiao XU
;
Miaoxian DONG
- Keywords:
Zhen-Gan Xi-Feng decoction;
6-hydroxydopamine;
reactive oxygen species;
Nfe2l2;
antioxidant response element;
drug-containing serum
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(3):486-490
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study the protective effects and related mechanisms of Zhen-Gan Xi-Feng (ZGXF) decoction containing serum on 6-OHDA-induced oxidative stress in PC12 cells.The ZGXF decoction containing rat serum with low-,medium-,and high-dose (8,16,or 32 g.kg-1) or blank serum was used to preprocess PC12 cells for 1 h,and cultured together with 100 μM 6-OHDA for 24 h.And then,cells were collected.The fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) and fluorescence microplate reader were used to detect the level of reactive oxygen species (ROS).Real-time quantitative PCR was used to analyze the mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2),Nfe2l2,heme oxygenase-1 (HO-1),glutamate-cysteine ligase catalytic subunit (GCLc),and GCL modulatory subunit (GCLm).The luciferase report gene system was used to detect the antioxidant response element (ARE) activation.The results showed that ZGXF decoction-containing serum inhibited the 6-OHDA-induced oxidative stress,upregulated the Nfe2l2,HO-1 and GCLc mRNA expressions in cells processed with 6-OHDA.However,it has no significant effect on GCLm mRNA expression.It was concluded that ZGXF decoction-containing serum had protective effects on 6-OHDA-induced oxidative stress in PC 12 cells.Its mechanism may be correlated with the upregulation on Nfe2l2 mRNA expression,which activated ARE and further induced its downstream gene of phase Ⅱ detoxifying enzyme,as well as the HO-1 and GCLc mRNA expressions of antioxidant enzyme gene.
