Radioimmunoimaging of 131I Labeled Human scFv Against Anaplastic Thyroid Carcinoma in Tumor Bearing Nude Mice
10.3969/j.issn.1005-5185.2017.05.001
- VernacularTitle:131I标记的甲状腺未分化癌人源单链抗体在荷瘤裸鼠模型的放射免疫显像
- Author:
Qian LIU
;
Qiong LIU
;
Wenbo LI
;
Lu XU
;
Jing ZHOU
;
Ying LIU
;
Hua PANG
- Keywords:
Thyroid neoplasms;
Carcinoma;
Iodine radioisotopes;
Single-chain antibodies;
Radioimmunodetection;
Tomography,emission-computed,single-photon;
Tomography,X-ray computed;
Disease models,animal;
Mice,nude
- From:
Chinese Journal of Medical Imaging
2017;25(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the radioactive purity and activity of 131I labeled human single chain variable fragments antibodies (scFv) against anaplastic thyroid carcinoma (ATC),and to explore its distribution and radioimmunoimaging characteristics in tumor bearing nude mice model in vivo so as to provide a new method for anaplastic thyroid carcinoma diagnosis and treatment.Materials and Methods The nude mice model bearing human anaplastic thyroid carcinoma was constructed.The chloramine T method was used to label scFv with 131I and the Sephadex G25M was used for purification of labeled scFv.Labeling rate was determined by trichloroacetic acid method;radiochemical purity,room temperature stability and serum stability were examined using paper chromatography.131I-scFv was injected via tail vein in mice,and the distribution of 131I-scFv in body tissues and organs was analyzed at 12,24,48,72 h after injection.Static SPECT imaging was performed at 12,24,48,72 h after injection to observe the intratumoral accumulation of radioactivity.The SPECT/CT image fusion was performed when the tumor tissues were clearly visible.Results 131I-scFv was purified,and the labeling rate was 91.64％;the radiochemical purity was (93.3 ±0.3)％.The radiochemical purity of 131l-scFv placed at room temperature and the serum for 1,6,12,24 h were all ＞90％.The radioactive distribution of 131I-scFv in tumor,liver,kidney,intestine and blood was high.SPECT imaging showed 131I-scFv was selectively concentrated in tumor tissue;the target/non-target ratio was the highest at 48 h,and the imaging was most satisfactory.Conclusion 131I-scFv can be successfully prepared.SPECT imaging of 131I-scFv in nude mice model is satisfactory,which lays the foundation for further research in ATC diagnosis and treatment.
