Expression miRNAs in cancer associated fibroblasts of gastric cancer and their effects on the migration and invasion
10.13315/j.cnki.cjcep.2017.06.003
- VernacularTitle:胃癌相关纤维母细胞中miRNAs的表达及对癌细胞迁移和侵袭的影响
- Author:
Yu YAN
;
Ruifen WANG
;
Meng QIAO
;
Lifeng WANG
- Keywords:
gastric neoplasms;
cancer associated fibroblasts;
miRNA-214;
invasion;
migration
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(6):601-605
- CountryChina
- Language:Chinese
-
Abstract:
To investigate various microRNAs (miRNAs) expression levels in cancer associated fibroblasts (CAFs) of gastric cancer,and to explore the effects of miRNA214 in CAFs on the invasion and migration of gastric cancer cells.Methods The primary CAFs were isolated from human gastric tumor tissues,while the normal fibroblasts (NFs) were from adjacent normal gastric mucosa tissues.Real-time quantitative PCR (RT-PCR) was performed to detect the expression level of miRNAs in CAFs and NFs.The interaction model in vitro between CAFs and MGC-803 (a gastric cancer cell line) was established by Transwell chamber,and then the effects of miRNA-214 in CAFs on MGC-803 invasion and migration were analyzed.Results It was found that the expression level of 18kinds of miRNAs in CAFs was different.Moreover,the expression level of miRNA-214 in CAFs was most observably decreased compared with NFs.Further studies indicated that over-expression of miRNA-214 in CAFs could markedly inhibit the migration and invasion ability of gastric cancer cells in vitro.Conclusion The expression of miRNAs was different between CAFs and NFs,and the miRNA-214 expression in CAFs is markedly reduced compared with NFs.The over-expression of miRNA-214in CAFs could significantly inhibit the migration and invasion ability of gastric cancer cells.Therefore,miRNA-214 in CAFs might act as a tumor suppressor in gastric cancer progression.
