Dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time
10.3969/j.issn.1001-1528.2017.05.027
- VernacularTitle:5个采收期女贞子中5种成分的动态变化
- Author:
Fucheng WANG
;
Donghan JI
;
Chengwu FANG
;
Daofeng GONG
;
Chengkai XIA
- Keywords:
Ligustri lucidi Fructus;
salidroside;
tyrosol;
luteolin-7-O-glucoside;
ligustroflavone;
specnuezhenide;
picking time;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(5):1011-1014
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time (August,September,October,November,December).METHODS The HPLC analysis of Ligustri lucidi Fructus ethanol extract was performed on a 25 ℃ thermostatic Aglient Zorbax SB-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 224 nm.RESULTS Salidroside,tyrosol,luteolin-7-O-glucoside,ligustroflavone and specnuezhenide showed good linear relationships within their own ranges (r ＞0.999 0),whose average recoveries were 99.56％-100.30％ with the RSDs of 0.89％-1.23％.The contents of various constituents (except for tyrosol) were the highest in samples picked up in September,followed by those picked up in October.CONCLUSION The suitable picking time of Ligustri lucidi Fructus is September and October.
