The protective effect of Arginase inhibitor on retinal microvascular endothelial cells in high glucose cultures
10.3760/cma.j.issn.1005-1015.2017.03.014
- VernacularTitle:精氨酸酶抑制剂对体外高糖培养的恒河猴视网膜微血管内皮细胞的保护作用
- Author:
Wei ZHANG
;
Jianhong JIANG
;
Song CHEN
;
Guanghui HE
;
Jing YANG
;
Yingxue MA
;
Li CHEN
;
Jian SONG
- Keywords:
Arginase/antagonists & inhibitors;
Retinal Vessels/cytology;
Endothelial cells/physiology;
Cells,cultured
- From:
Chinese Journal of Ocular Fundus Diseases
2017;33(3):281-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arginase (Arg) inhibitor N-ω-Hydroxy-L norArginine (nor-NOHA) on high glucose cultured rhesus macaque retinal vascular endothelial cell line (RF/6A) in vitro.Methods The RF/6A cells were divided into the following 4 groups:normal control group (5.0 mmol/L of glucose,group A),high glucose group (25.0 mmol/L,group B),high glucose with 125 mg/L nor-NOHA group (group C),and high glucose with 1％ DMSO group (group D).The proliferation,migration ability and angiogenic ability of RF/6A cells were measured by Methyl thiazolyl tetrazolium (MTT),transwell chamber and tube assay respectively.The express of Arg Ⅰ,eNOS,iNOS mRNA of RF/6A cells were measured by real-time polymerase chain reaction (RT-PCR),Enzyme-linked immuno sorbent assay (ELISA) was used to detect the expression of NO and interleukine (IL)-1b of RF/6A cells.Results The proliferation,migration,and tube formation ability of group A (t=2.367,5.633,7.045;P＜0.05) and group C (t=5.260,6.952,8.875;P＜0.05)were significantly higher than group B.RT-PCR results showed the Arg Ⅰ and iNOS expression in group B was higher than that in group A (t=6.836,3.342;P＜0.05) and group C (t=4.904,7.192;P＜0.05).The eNOS expression in group B was lower than that in group A and group C (t=4.165,6.594;P＜0.05).ELISA results showed NO expression in group B was lower than that in group A and group C (t=4.925,5.368;P＜0.05).IL-1b expression in group B was higher than that in group A and group C (t=5.032,7.792;P＜0.05).Conclusions Nor-NOHA has a protective effect on cultured RF/6A cells in vitro and can enhance its proliferation,migration and tube formation.The mechanism may be inhibiting the oxidative stress by balancing the expression of Arg/NOS.
