Effect of venous marker COUP-TFⅡ knockdown on senescence of vascular endothelial cells
10.3969/j.issn.1000-4718.2017.08.001
- VernacularTitle:静脉表型分子COUP-TFⅡ表达降低对血管内皮细胞衰老的影响
- Author:
Yayu LAI
;
Qi ZHOU
;
Wei ZHENG
;
Peng JIN
;
Wenzhu GU
;
Xiaojing WU
- Keywords:
Atherosclerosis;
Vascular endothelial cells;
Cell senescence;
Cell proliferation;
Chicken ovalbumin upstream promoter-transcription factor Ⅱ
- From:
Chinese Journal of Pathophysiology
2017;33(8):1345-1352
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of venous marker chicken ovalbumin upstream promoter-transcription factor Ⅱ (COUP-TFⅡ) expression on vascular endothelial cell senescence and its molecular mechanism.METHODS: The mRNA expression of COUP-TFⅡ in the human coronary artery endothelial cells (HCAEC) and human umbilical vein endothelial cells (HUVEC) was detected by RT-qPCR.After transfection with a COUP-TFⅡ siRNA (siCOUP-TFⅡ) to inhibit COUP-TFⅡ expression in the HUVEC, the senescence and proliferation of endothelial cells were evaluated by β-galactosidase staining, Western blot, CCK-8 assay and cell counting after treatment with 10-5 mol/L angiotensin Ⅱ (AngⅡ).The protein levels of Akt and p-Akt were determined by Western blot.RESULTS: Compared with the HCAEC, COUP-TFⅡ was significantly highly expressed in the HUVEC.Knockdown of COUP-TFⅡ via siCOUP-TFⅡ significantly induced endothelial cell senescence and inhibited endothelial cell proliferation and p-Akt level after treatment with AngⅡ at 10-5 mol/L.Furthermore, an Akt activator SC79 at 4 mg/L partly reversed the effect of siCOUP-TFⅡ on AngⅡ-induced endothelial cell senescence and proliferation.CONCLUSION: Knockdown of COUP-TFⅡ promotes endothelial cell senescence and inhibits endothelial cell proliferation, which might be partly regulated by Akt signaling.
