Effects of oridonin on invasion and migration of human hepatocellular carcinoma MHCC-97H cells
10.3969/j.issn.1000-4718.2017.08.013
- VernacularTitle:冬凌草甲素对人肝癌MHCC-97H细胞系侵袭和迁移的作用
- Author:
Chunyu LI
;
Qi WANG
;
Shen SHEN
;
Weilian SU
;
Jingxiu LI
;
Guoxia LI
- Keywords:
Oridonin;
Hepatocellular carcinoma;
Cell invasion;
Cell migration;
Cell adhesion
- From:
Chinese Journal of Pathophysiology
2017;33(8):1423-1427
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of oridonin on the invasion and migration of hepatocelluar carcinoma cells.METHODS: Human hepatocelluar carcinoma MHCC-97H cells were cultured and treated with 5, 10 or 20 μmol/L oridonin.The migration ability was measured by wound healing assay.The invasion ability was examined by Transwell invasion assay.The adhesion capabilities were evaluated by adhesion assay.The protein levels of LIM kinase-1 (LIMK-1), cofilin and phosphorylated cofilin (p-cofilin) were determined by Western blot.RESULTS: Oridonin inhibited the migration, invasion and adhesion abilities of MHCC-97H cells in a dose-dependent manner (P<0.05).After oridonin treatment, the expression of cofilin had no obvious change, but the protein levels of LIMK-1 and p-cofilin decreased significantly.CONCLUSION: Oridonin inhibits the invasion and migration of MHCC-97H cells.The mechanism may be related with the regulatory effect of oridonin on LIMK-1/cofilin signal transduction pathway.
