Effects of Periploca forrestil Schltr Compound extracts on IL-4,IFN-γ and TGF-β1 of rats with adjuvant arthritis
10.3969/j.issn.1000-484X.2017.08.009
- VernacularTitle:复方黑骨藤对佐剂性关节炎大鼠IL-4、IFN-γ及TGF-β1表达的影响
- Author:
Ran RAN
;
Yuting HOU
;
Xia LI
- Keywords:
Periploca forrestil Schltr Compound;
Rheumatoid arthritis;
Th1/Th2;
Interleukin-4;
Interferon-γ;
Transforming growth factor-β1
- From:
Chinese Journal of Immunology
2017;33(8):1164-1169,1176
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Periploca forrestil Schltr Compound extract in serum interleukin-4(IL-4),interferon-γ(IFN-γ),tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in joint synovial tissue of rats,and further to investigate it′s therapeutic effects and related mechanism.Methods: To built the complete adjuvant arthritis (AA) model in rats,and then to detect the contents of IL-4,IFN-γ,TNF-α in serum by ELISA,TGF-β1 in synovial tissues of rats knee was detected by immunohistochemistry.Results: The contents of IFN-γ,TNF-α were higher and IL-4 and TGF-β1 were lower in adjuvant arthritis rats (model group) than those in blank control (P<0.01).By comparing with the model group the contents of IFN-γ and TNF-α were significantly lower in Periploca forrestil Schltr Compound treated rat group,whereas the expressions of IL-4 and TGF-β1 were increased(P<0.05).Conclusion: The Periploca forrestil Schltr Compound can significantly inhibit the expression of IFN-γ and TNF-α,and enhance the expression of IL-4 and TGF-β1,adjusted the Th1/Th2 cell and cytokines imbalance,to alleviate arthritis and cartilage destruction,therefore the Periploca forrestil Schltr Compound can significantly inhibit the proliferation of T lymphocyte and infiltration in synovial tissues of experimental rheumatoid arthritis.
