Promoting effect of Ginsenoside Ro on differentiation of THP-1-derived DC
10.3969/j.issn.1000-484X.2017.08.010
- VernacularTitle:人参皂苷Ro促进THP-1细胞向DC分化的作用
- Author:
Ziwei LI
;
Ning YU
;
Xiaoxiao ZHU
;
Lin ZHAO
;
Bo ZHANG
;
Qiang GUO
;
Zhen ZHANG
;
Ran WEI
;
Na DING
;
Xia LI
- Keywords:
Ginsenoside Ro;
Leukemia;
Dendritic cells;
THP-1 cells;
GM-CSF
- From:
Chinese Journal of Immunology
2017;33(8):1170-1176
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the promoting effect of Ginsenoside Ro on the differentiation of THP-1-derived dendritic cells (DCs) induced by GM-CSF and IL-4.Methods: Sensitive leukemia-derived DC cell line was screened first.Then,the selected sensitive cell line THP-1 was stimulated to differentiate into DCs by cytokines (GM-CSF and IL-4) and small(5 μmol/L),middle(10 μmol/L),and large (20 μmol/L) dose of Ginsenoside Ro respectively.The expressions of CD1a,MHCⅡ and CD86 of leukemia-derived DCs were detected by flow cytometry.In addition,the transcription levels of CD1a,CD86 and MHCⅡ of leukemia-derived DCs were detected by RT-PCR.ELISA was used to measure the protein levels of TNF-α and IL-6 in the culture supernatant.Results: THP-1 was the sensitive leukemia cell line which could be induced to differentiate into DCs by cytokines.Compared with cytokine stimulation alone,the expression of CD1a,MHCⅡ and CD86 in leukemia-derived cells was significantly increased after the stimulation of Ginsenoside Ro combined with cytokine(P<0.05).The CD1a,CD86 and MHCⅡ mRNA expression was significantly increased after the treatment of Ginsenoside Ro combined with cytokine(P<0.05).Moreover,the protein levels of TNF-α and IL-6 in culture supernatant were significantly increased (P<0.05) after the stimulation of Ginsenoside Ro in combination with cytokines.Conclusion: Ginsenoside Ro can significantly promote the differentiation of leukemia-derived DCs.
