Puerarin attenuates the expressions ofHUVECs tissue factor and its inhibitor induced by ox-LDL
- VernacularTitle:葛根素减弱ox-LDL诱导HUVECs组织因子及其抑制物的表达
- Author:
Huafei DENG
;
Jian LI
;
Qin ZHOU
;
Yulin TAN
;
Ming XIE
;
Ying HAN
;
Wanbei GUO
- Keywords:
puerarin;
ox-LDL;
tissue factor;
tissue factor pathway inhibitor;
endothelial cells
- From:
Basic & Clinical Medicine
2017;37(8):1094-1097
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of puerarin on the expression of human umbilical vein endothelial cells (HUVECs) tissue factor (TF) and tissue factor pathway inhibitor (TFPI) induced by oxidized low-density lipoprotein (ox-LDL).Methods After HUVECs were incubated with different concentrations of puerarin and 50 mg/L ox-LDL,the expression of TF and TFPI mRNA and protein were detected by real-time fluorescent quantitative PCR and Western blot respectively.Results Compared with control,treatment with ox-LDL caused the augment of TF mRNA and protein expression (P<0.01),and the decrease of TFPI mRNA and protein expression.However,50,100,and 200 μmol/L puerarin blunted the augment of TF mRNA and protein expression and weakened the inhibition of TFPI mRNA and protein expression induced by ox-LDL(P<0.01).Conclusions Puerarin reduces HUVECs TF and TFPI mRNA and protein induced by ox-LDL.
