Effects of caveolin-1 scaffolding domain peptide on LPS-induced acute lung injury in mice
10.3969/j.issn.1000-4718.2017.08.021
- VernacularTitle:小窝蛋白1脚手架区多肽减轻LPS诱导的小鼠急性肺损伤
- Author:
Ping WENG
;
Xiaotong ZHANG
;
Wei CHEN
;
Wenfang TIAN
;
Junliang CHEN
;
Jiajia YUAN
;
Xinjie CHEN
;
Qingfeng PANG
- Keywords:
Caveolin-1;
Heme oxygenase-1;
Acute lung injury;
Lipopolysaccharide
- From:
Chinese Journal of Pathophysiology
2017;33(8):1475-1480
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of caveolin-1 (Cav-1) scaffolding domain peptide, cavtratin, on lipopolysaccharide (LPS)-induced mouse acute lung injury and heme oxygenase-1 (HO-1) activity.METHODS: Adult male BALB/c mice were randomly divided into 6 groups (n=8 to 10): control, Antennapedia internalization sequence (AP), LPS, LPS+hemin, LPS+ hemin+cavtratin and LPS+hemin+cavtratin+zinc protoporphyrin IX (ZnPP) groups.After LPS administration for 24 h, the lung pathological changes, the wet/dry weight (W/D) ratio of lung tissues, total cell number in bronchoalveolar lavage fluid and serum lactate dehydrogenase activity were measured.The co-localization of HO-1 and Cav-1 was displayed by immunofluorescence, and the HO-1 activity were detected.The mRNA expression of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, MCP-1 and iNOS was detected by real-time PCR.RESULTS: The mice in LPS+hemin+cavtratin group had the decreased interaction between HO-1 and Cav-1, and the increased HO-1 activity compare with LPS group (P<0.05).Compared with LPS group, the pulmonary damage was attenuated in LPS+hemin+cavtratin group, and the injury indexes, including W/D ratio, total cell number in bronchoalveolar lavage fluid and lactate dehydrogenase activity in the serum, and the mRNA expression of inflammatory cytokines all decreased (P<0.05).HO-1 activity inhibitor ZnPP abolished the above protective effect of cavtratin on the lung tissues with LPS-induced acute lung injury.CONCLUSION: Cavtratin has beneficial effects on the lung with LPS-induced acute injury by restoring the HO-1 activity.
