Role of SIRT3 in dysfunction of energy metabolism induced by deoxycho-lic acid in human colon NCM460 cells
10.3969/j.issn.1000-4718.2017.08.024
- VernacularTitle:SIRT3在脱氧胆酸诱导人正常结肠上皮NCM460细胞能量代谢损伤中的作用
- Author:
Chuanjie WANG
;
Yang ZHOU
;
Meng ZHANG
;
Ying ZHOU
;
Jiaqi XU
;
Minhang ZHU
;
Lin ZHAN
;
Qianyi ZHOU
;
Qiong YUAN
- Keywords:
Deoxycholic acid;
Energy metabolism;
Lactate acid;
Adenosine triphosphate;
Sirtuin 3;
Resvertrol
- From:
Chinese Journal of Pathophysiology
2017;33(8):1494-1498
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of deoxycholic acid (DCA) on the energy metabolism in human normal colon epithelial NCM460 cells.METHODS: NCM460 cells was treated with DCA at 10, 30 and 100 μmol/L for 5 d, or DCA at 100 μmol/L for 3, 5 and 7 d.After treated with DCA at 100 μmol/L for 3 d, the cells were treated with resveratrol, the activator of sirtuin 3 (SIRT3), for the next 4 d.Adenosine triphosphate (ATP) production in the mitochondria and lactate acid level were detected.The protein expression of SIRT3 was determined by Western blot.RESULTS: DCA inhibited the ATP production, increased lactate acid level, and downregulated the protein expression of SIRT3 in a dose-and time-dependent manner.Resveratrol at 10 μmol/L reversed the effects of DCA on the NCM460 cells.CONCLUSION: DCA induces the dysfunction of energy metabolism in NCM460 cells, and the mechanism may be related with SIRT3.
