Effect of GYY4137 on cytosolic lipid decomposition in mouse primary hepatocytes
10.3969/j.issn.1000-4718.2017.08.028
- VernacularTitle:GYY4137对小鼠原代肝细胞胞质内脂质分解的影响
- Author:
Honggang WANG
;
Youjing ZHANG
;
Chaoshen HUANGFU
;
Jun WANG
;
Dongdong WU
;
Peiyu ZHONG
;
Guoying WANG
;
Yanzhang LI
- Keywords:
Hydrogen sulfide;
Cytosolic lipid decomposition;
Hepatocytes;
Oleic acid
- From:
Chinese Journal of Pathophysiology
2017;33(8):1520-1523
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the effect of GYY4137, a novel hydrogen sulfide (H2S) donor, on cytosolic lipid decomposition in mouse primary steatosis hepatocytes.METHODS: Oleic acid (OA) was used to induce hepatic steatosis model in vitro.The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion were divided into 4 groups: the cells in control group were incubated with normal medium for 54 h;the cells in model group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 for 6 h;the cells in H2S group or DL-propar-gylglycine (PAG;an inhibitor of cystathione γ-lysase, inhibiting H2S synthesis) group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h.The glycerin release and the protein expression of hormone-sensitive lipase (HSL) in the cells were mea-sured.RESULTS: Compared with model group, the glycerin release and the protein expression of phosphorylated HSL (p-HSL) in H2S group decreased significantly, while those increased significantly in PAG group.CONCLUSION: In steatosis hepatocytes, exogenous H2S possibly decreases cytosolic lipid decomposition by decreasing the protein level of p-HSL.
