A comparative study on three methods of enzyme linked immunosorbent assay for detecting hepatitis B virus Pre S1 and S2 antigen

VernacularTitle:3种酶联免疫吸附试验检测乙型肝炎病毒前S1及S2抗原的比较
Author: Jie FU ; Guojun YANG ; Xingyu JIANG ; Xiaoyun PU
Keywords: enzyme linked immunosorbent assay; precision; sensitivity
From: International Journal of Laboratory Medicine 2017;38(15):2017-2018,2021
CountryChina
Language:Chinese
Abstract: Objective To compare the three different methods of enzyme linked immunosorbent assay(ELISA),to select the best method for clinical diagnosis and treatment.Methods Addcare ELISA800,TECAN freedom evolyzer and manual ELISA method were used to detect hepatitis B virus Pre S1 antigen(preS1Ag) hepatitis B virus Pre S2 antigen(preS2Ag) in confrontation control product samples and serum specimens from patients with HBV,and the results were analyzed by statistical methods.Results The batch precisions of the three methods to detect pre-S1Ag were 4.73%,5.38%,11.87%,the batch precisions of the three methods to detect pre-S2Ag were 4.91%,5.04%,11.75%.The inter batch precisions of the three methods to detect pre-S1Ag were 6.63%,7.90%,13.26%,the inter batch precisions of the three methods to detect pre-S2Ag were 6.74%,7.81%,12.59%.All the sensitivities were 100.00%.Conclusion All the three methods have good consistency,which could be used in the detection of Pre-S1Ag and Pre-S2Ag.The precision of Addcare ELISA800 is the best,which could further improve the quality of clinical testing.