Research on screening carbapenemase-producing Enterobacteriaceae by modified Hodge test
10.3969/j.issn.1673-4130.2017.15.007
- VernacularTitle:应用改良Hodge试验检测肠杆菌科细菌产碳青霉烯酶的研究
- Author:
Chunbao XIE
;
Jiangrong LUO
;
Liangmin CHUAN
;
Daiwen XIAO
;
Hua YU
;
Yongchang YANG
;
Wei JIANG
;
Wenfang HUANG
- Keywords:
Enterobacteriaceae;
carbapenemase;
modified Hodge test;
polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2017;38(15):2034-2035,2039
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the application value of modified Hodge test(MHT) for screening carbapenemase-producing Enterobacteriaceae.Methods The 24 Enterobacteriaceae reduced susceptibility to carbapenems were detected by MHT.At the same time,polymerase chain reaction(PCR) was used to detect carbapenemase genes of KPC,NDM,IMP,SIM and VIM.PCR products were sequenced and the results were compared with the sequences of Gen Bank database.Comprehensive analysis the application value of MHT and PCR to detect carbapenemase.Results Among these 24 strains,13 stains appeared to produce carbapenemase by MHT,5 positive strains were found to carry carbapenemase genes by PCR.By comparing with the sequences of Gen Bank database 1 strain were confirmed to KPC-2 and 4 strains were confirmed to IMP-4.We found that 4 strains of Enterobacteriaceae,detected carbapenemase by MHT and PCR at the same time.9 strains of MHT were positive,but we couldn′t detect the carbapenemase genes.1 strain of MHT was negative,but carbapenemase gene was found in the strain.Conclusion The value of MHT to screen carbapenemase-producing Enterobacteriaceae is necessary to further study.
