Foxp3 overexpression in lung cancer cells suppresses immune activities of activa-ted CD4+T cells
10.3969/j.issn.1000-484X.2017.08.004
- VernacularTitle:高表达Foxp3的肺癌细胞抑制人CD4+T细胞免疫活性
- Author:
Ruimin LIU
;
Aihong ZHANG
;
Weixia CHAO
;
Danni SUN
;
Mingli WANG
;
Yuanfang MA
;
Huiling BAI
- Keywords:
Foxp3;
Lung cancer;
IL-8;
IL-10;
CD4+T lymphocyte;
Immune activities
- From:
Chinese Journal of Immunology
2017;33(8):1141-1145
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effects of Foxp3-overexpressing lung cancer cells on activated CD4+T lymphocyte.Methods: Stable Foxp3-overexpressing lung cancer cells NCIH-1299,NCIH-hFoxp3,was generated by transfection of NCIH-1299 cells with plasmid pcDNA3-hFoxp3 mediated by Lipofectamine 2000 and by selection with G418,and validated by quantitative PCR and Western blot.The expression levels of IL-8 and IL-10 secreted by NCIH-hFoxp3 and NCIH-control were measured by ELISA.IL-2 secrection by activated human CD4+T lymphocyte which was tested after stimulation with 20% conditioned medium of NCIH-hFoxp3 and NCIH-control cells.The proliferation of activated human CD4+ T lymphocytes was assessed by MTT after coculture with NCIH-hFoxp3 cells.The adhesive ability of activated human CD4+ T lymphocytes was probed with NCIH-hFoxp3 cells by immunocytochemistry.Results: Compared with NCIH-control cells,NCIH-hFoxp3 secreted high level of IL-10 and low level of IL-8.NCIH-hFoxp3 with Foxp3 overexpression significantly suppressed the proliferation,adhesive potential and IL-2 expression by activated CD4+ T cells.Conclusion: Suppression of immune activities of activated CD4+ T cells by Foxp3 overexpression in lung cancer cells may correlate with cytokine IL-8 and IL-10,which can contribute lung cancer progression.
