miR-10b overexpressionpromotes the proliferation of lung cancer cell line A549
- VernacularTitle:过表达miR-10b促进肺癌细胞系A549增殖
- Author:
Jing TANG
;
Zhongquan SHI
;
Ling HU
;
Guoli LI
;
Xiaoling FENG
;
Feng LIU
- Keywords:
miR-10b;
Kruppel-like factor 4;
non-small cell lung cancer;
proliferation;
malignant
- From:
Basic & Clinical Medicine
2017;37(8):1108-1112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the of miR-10b cordribution in patients with non-small cell lung cancer (NSCLC) and adjacent tissues,and to investigate the effect of miR-10b on the malignant change of lung cancer cell A549 by regulating the expression of Kruppel-like factor 4 (KLF4).Methods Fourty patients with NSCLC were selected with lung cancer and in miR-10b expression adjacent tissues of lung cancer cell A549 transfected miR-10b mimics,changes of CCK-8 assay were used to detect the proliferation of lung cancer cells;real time PCR and Western blot were used to examine the cell KLF4 mRNA and protein levels;soft agar colony formation assay was used to detect the expression of miR-10b on the proliferation of lung cancer cell A549 tumor malignant.Results miR-10b expression in lung cancer A549 cells and lung cancer tissue were higher than that of normal lung epithelial 16HBE cells and cancer adjacent tissues;overexpression of miR-10banalogue in A549 cells,KLF4 protein levels significantly decreased,KLF4 mRNA was not changed significantly;miR-10b expression significantly increased the growth of A549 cells.Conclusions The distribution of miR-10b in different cell types and tissues may be different,which may promote the proliferation and malignancy of lung cancer cells by inhibitingthe expression of KLF4.
