Over-expressed miR-449a inhibitsproliferation and induces apoptosis of SH-SY5Y cells in vitro
- VernacularTitle:miR-449a过表达抑制人神经母细胞瘤细胞SH-SY5Y增殖并促进其凋亡
- Author:
Xiaoyan XU
;
Xiaoshan LI
- Keywords:
miRNA-449a;
SH-SY5Y;
proliferation;
apoptosis;
cell cycle
- From:
Basic & Clinical Medicine
2017;37(8):1117-1121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of miR-449a on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells.Methods Transferred miR-449a mimics SH-SY5Y cells by LipofectamineTM2000 as experimental group.Cell growth was measured by CCK8 assay,the cell cycle and apoptosis were detected by flow cytometry.Relative expression of c-Myc and Bax/Bcl-2 was detected by Western blot.Results Compare to blank and negative control cells,the proliferation of miR-449a mimics SH-SY5Y cells was decreased(P<0.05).Flow cytometry showed that over-expressed miR-449a resulted in an increase of G0/G1 phase and apoptotic cells.The expression of c-Myc showed significant difference (P<0.05),Expression of pro-apoptotic protein Bax was increased,and that of anti-apoptotic protein Bcl-2 was decreased(P<0.05).Conclusions miR-449a over-expression can inhibit cell proliferation by regulating cell cycles and induce apoptosis mainly through regulating Bcl-2 family proteins expression in SH-SY5Y cells.
