Study on the diagnostic value of serum miRNA-182 in colorectal cancer
10.3969/j.issn.1673-4130.2017.15.004
- VernacularTitle:血清miRNA-182检测在结直肠癌中的诊断价值研究
- Author:
Yan ZHANG
;
Lin CHEN
;
Feng WANG
- Keywords:
colorectal cancer;
miRNA-182;
serum biomarker;
diagnosis
- From:
International Journal of Laboratory Medicine
2017;38(15):2025-2027
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of serum miRNA-182 in colorectal cancer(CRC).Methods A total of 53 CRC patients were enrolled in this study,serum miRNA-182 was detected by quantitative real time polymerase chain reaction(qRT-PCR).The results were compared with those of patients with benign intestinal diseases and healthy controls.Results The content of serum miRNA-182 in CRC patients was significantly higher than those in patients with benign intestinal diseases and healthy group(P<0.05).The ROC curve showed that the area under the curve of serum miRNA-182 was 0.846,the confidence interval was 0.768-0.925,the diagnostic sensitivity was 77.1%,and the specificity was 81.1%.Serum miRNA-182 levels were correlated with TNM stage(P=0.011) and distant metastasis(P=0.002) in patients with CRC,but no significant correlation with other clinical parameters(P>0.05).Serum miRNA-182 level in CRC patients after surgery was significantly lower than that before operation(P<0.01),and which was significantly higher in patients with recurrence/metastasis after surgery(P=0.019).Conclusion Serum miRNA-182 level increased significantly in CRC patients,and which was closely related to the disease and malignant progression,serum miRNA-182 could be used as a potential marker for clinical diagnosis and disease evaluation of CRC.
