Study on effect mechanism of sanguinarine on proliferation, apoptosis, invasion and migration of cervical cancer cells
10.3969/j.issn.1671-8348.2017.22.005
- VernacularTitle:血根碱对宫颈癌细胞增殖、凋亡、侵袭、迁移能力影响机制研究
- Author:
Zhencai LI
;
Ping JIANG
;
Qiuyu WANG
;
Li YANG
;
Pengxiao FU
;
Jinru ZHANG
- Keywords:
uterine cervical neoplasms;
sanguinarine;
invasion;
migration;
proliferation
- From:
Chongqing Medicine
2017;46(22):3039-3042
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect mechanism of sanguinarine on the proliferation,apoptosis,invasion and migration abilities of cervical cancer cells.Methods MTT,flow cytometer,cell scratch test and Transwell chamber assay were respectively used to detect the cellular proliferation,apoptosis,migration and invasion abilities after sanguinarine action.The expression levels of E-Cadherin,PTEN,β-catenin and MMP2 protein of cervical cancer cells after sanguinarine action were detected by Western blot.Results 0.6,0.8 μmol/L sanguinarine had the inhibitory effect on the proliferation of cervical cancer cells.After 0.8μmol/L sanguinarine action for 48 h,cervical cancer HeLa and Siha cells apoptosis rate were up to (45.68± 2.26)％ and(31.89 ± 3.80)％ respectively.0.8 μmol/L sanguinarine action for 3 h,cervical cancer cells HeLa and Siha adhesion rates were only (67.45 ± 2.13)％and(73.59± 2.61)％.0.8 mol/L sanguinarine action for 16 h,the invasion numbers of cervical cancer Hela and Siha cell were (39.64 ±1.98) and (43.87 ± 2.83) respectively.The expression amount of E-Cadherin and PTEN in cervical cancer cells after sanguinarine action was increased,while the expression amount of E-Cadherin and PTEN was weakened.Conclusion Sanguinarine has the proliferation inhibiting and apoptosis promoting effect on cervical cancer cells,its mechanism may be related to adhesion protein E-Cadherin,β-catenin and PTEN,MMP2.
