Mechanism of action of miRNA-146a in sepsis-induced acute lung injury
10.11855/j.issn.0577-7402.2017.03.06
- VernacularTitle:miRNA-146a在脓毒症引起的急性肺损伤中的作用机制
- Author:
Jianbin MENG
;
Liping SUN
;
Yonglu WA
;
Lili LIU
;
Qian YAO
- Keywords:
miRNA-146a;
sepsis;
acute lung injury
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(3):207-210
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the expression of miRNA-146a in sepsis-induced acute lung injury (ALI) patients and its effect on the inflammation in mouse models.Methods miRNA-146a expression in peripheral blood was determined in sepsisinduced ALI patients and healthy volunteers by qRT-PCR.Sepsis-induced ALI mouse model were reproduced by LPS treatment and miRNA-146a mimic,miRNA-146a NC and miRNA-146a inhibitors were injected through trachea.The expressions of miRNA-146a,TNF-α,IL-1β and COX-2 mRNA were determined by qRT-PCR 24h after the intervention.Results The miRNA-146a expression in peripheral blood significantly increased in severe sepsis with ALI patients,compared with the control subjects.For mouse model,the expressions of TNF-o,IL-1β and COX-2 mRNA significantly decreased in miRNA-146a group,while increased in miRNA-146a inhibitor group compared with miRNA-146a NC group (P＜0.05).The TNF-α and IL-1β expressions significantly decreased in miRNA-146a inhibitor+COX-2 inhibitor group compared with miRNA-146a inhibitor group (P＜0.05).Conclusion MiR-146a treatment can effectively alleviate the lung inflammation in sepsis-induced ALI mice.
