Research on Hydrogen Peroxide Induced Degradation of Human Articular Chondrocyte Extracellular Matrix in Vitro
- VernacularTitle:过氧化氢体外诱导人关节软骨细胞外基质降解研究
- Author:
Peng YANG
;
Xiaoqing HU
;
Xin FU
;
Qiang LIU
;
Jiying ZHANG
;
Xiaoning DUAN
;
Yingfang AO
- Keywords:
hydrogen peroxide;
articular chondrocyte;
extracellular matrix;
osteoarthritis
- From:Chinese Journal of Sports Medicine
2017;36(4):306-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of elevating the oxygen pressure on articular chondrocytes in vitro.Method A hydrogen peroxide induced human articular chondrocyte damage model was established.Then the articular chondrocyte viability was detected using the CCK-8 kit.Collagen Ⅱ(COL Ⅱ),The expression levels of aggrecan (ACAN),matrix metalloproteinase 13 (MMP13) and adisintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) were detected using the realtime PCR and Western blotting.Result The viability of articular chondrocytes improved at 12 h but decreased at 24 h after the stimulation of hydrogen peroxide.Twenty-four hours later,the average expression level of COL Ⅱ and ACAN decreased(P＜0.05),while that of MMP13 and ADAMTS5 elevated(P＞0.05).Conclusion Hydrogen peroxide induced elevation of the extracellular oxygen pressure can influence the synthesis and degradation of the articular chondrocyte extracellular matrix.
