Pig bone marrow stromal cells transfected with human tissue factor pathway inhibitor combat species incompatibility and regulate the human TF pathway in vitro
10.3760/cma.j.issn.0254-1785.2016.12.004
- VernacularTitle:使用转人TFPI的猪骨髓间充质干细胞缓解异种凝血功能障碍的研究
- Author:
Xiao LI
;
Hongchen JI
;
Zhuochao ZHANG
;
Ben MA
;
Xuan ZHANG
;
Kaishan TAO
;
Kefeng DOU
- Keywords:
Xenotransplantation;
Coagulation dysregulation;
Tissue factor;
Tissue factor pathway inhibitor
- From:
Chinese Journal of Organ Transplantation
2016;37(12):720-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the reception of using pig bone marrow stromal cells (BMSCs) that were transfected with human tissue factor pathway inhibitor (TFPI) to resolve the dysregulation of coagulation after liver xenotransplantation.Methods Pig BMSCs were immortalized by transfection with lentivirus containing SV40T and then transfection with human TFPI.At last the cells were tested for their ability to inhibit clotting in a model by co-incubation of human plasma,human monocytes and pig aortic endothelial cells.Results After transfection with SV40T,pig BMSCs were immortalized and similar to primary cells.The immortalized pig BMSCs showed a stable TFPI expression after transfection with human TFPI by lentivirus.Moreover,they showed the potential of regulating coagulation dysregulation in vitro.Conclusion Pig BMSCs transfected with human TFPI could solve the regulation dysregulation caused by TF activation effectively,and have the potential of resolving coagulation dysregulation in liver xenotransplantation.
