Effect of tripterine on hydrochloric acid-induced acute lung injury in mice
10.3760/cma.j.issn.0254-1416.2017.03.032
- VernacularTitle:雷公藤红素对小鼠盐酸吸入性急性肺损伤的影响
- Author:
Juntao WANG
;
Juan LIU
;
Haichen CHU
;
Xiao ZHANG
;
Li YUAN
;
He DONG
- Keywords:
Tripterygium;
Hydrochloric acid;
Respiratory distress syndrome;
adult
- From:
Chinese Journal of Anesthesiology
2017;37(3):382-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of tripterine on hydrochloric acid-induced acute lung injury(ALI)in mice.Methods Eighteen pathogen-free healthy adult male ICR mice,aged 7-9 weeks,weighing 25-30 g,were divided into 3 groups(n=6 each)using a random number table:control group(group C),hydrochloric acid-induced ALI group(group ALI)and tripterine group(group T).ALI was induced by a single intratracheal instillation of hydrochloric acid 2 ml/kg(pH 1.5)via a 24-gauge angiocatheter inserted into the trachea in pentobarbital sodium-anesthetized mice.Tripterine 3 mg/kg was injected intraperitoneally once a day for 3 consecutive days,and then the model was established in group T.The mice were sacrificed at 6 h after instillation,and lung specimens were obtained for microscopic examination and for determination of the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),macrophage migration inhibitory factor(MIF)and myeloperoxidase(MPO)in lung tissues.Results Compared with group C,the levels of TNF-α,IL-6,MIF and MPO were significantly increased at 6 h after instillation in ALI and T groups(P<0.01).Compared with group ALI,the levels of TNF-α,IL-6,MIF and MPO were significantly decreased at 6 h after instillation in group T(P<0.01).The pathological changes of lung tissues were significantly attenuated in group T compared with group ALI.Conclusion Tripterine can attenuate hydrochloric acid-induced ALI in mice.
