Effect of hydrogen on activation of caspase-3 in brain tissues during cerebral ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2017.03.013
- VernacularTitle:氢对大鼠脑缺血再灌注时脑组织caspase-3活化的影响
- Author:
Feng JIN
;
Long HE
;
Yanqiu AI
;
Da LI
;
Wei ZHANG
- Keywords:
Hydrogen;
Caspase 3;
Reperfusion injury;
Brain
- From:
Chinese Journal of Anesthesiology
2017;37(3):305-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of hydrogen on the activation of caspase-3 in brain tissues during cerebral ischemia-reperfusion (I/R) in rats.Methods Thirty-six healthy adult male Sprague-Dawley rats,weighing 220-250 g,were divided into 3 groups (n=12 each) using a random number table:sham operation (group S),I/R group and hydrogen group (group H).Cerebral ischemia was induced by occlusion of the middle cerebral artery followed by reperfusion in I/R and H groups.In group H,hydrogen-rich saline 5 ml/kg (0.6 mmol/L) was injected intraperitoneally at 3 days before establishment of the model and immediately after the onset of reperfusion.At 24 h of reperfusion,the rats were sacrificed,and hippocampal tissues were obtained for determination of neuroapoptosis (by TUNEL),apoptotic neuron count and expression of activated caspase-3 (by Western blot).The brain tissues in the ischemic area were obtained and stained with haematoxylin and eosin for examination of the pathological changes.Results Compared with group S,the expression of activated caspase-3 was significantly up-regulated,and the apoptotic neuron count was increased in I/R and H groups (P<0.05).Compared with group I/R,the expression of activated caspase-3 was significantly down-regulated,the apoptotic neuron count was decreased (P<0.05),and the pathological changes of brain tissues were significantly reduced in group H.Conclusion The mechanism by which hydrogen inhibits neuroapoptosis during cerebral I/R is probably related to inhibited activation of caspase-3 in brain tissues of rats.
