Bone marrow mesenchymal stem cells in repairing donor liver after cardiac death for liver under normothermic machine perfusion in rats
10.3760/cma.j.issn.1007-8118.2017.04.011
- VernacularTitle:常温机械灌注下大鼠骨髓间充质干细胞修复心脏死亡后捐献供肝
- Author:
Mingli YIN
;
Yang YANG
;
Yuan SHI
;
Liu YANG
;
Raorao WANG
;
Zhongyang SHEN
;
Hongli SONG
- Keywords:
Liver transplantation;
Normothermic mechanical perfusion;
Mesenchymal stem cells;
Donation after cardiac death;
Warm ischemia;
Rats
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(4):259-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the repairing effect of bone marrow mesenchymal stem cells (BMMSCs) on the donor liver after cardiac death (DCD) under normal temperature mechanical perfusion (NMP) in rats.Methods BMMSCs of Wistar rats were cultured in vitro,and 45-min warm ischemia after cardiac death model was established.The 30 Wistar rats were randomly divided into NMP,NMP + BMMSCs (N + B),cold storage (CS) groups,and the parameters were detected at 2 h and 4 h (n =10).Results N + B group was superior to NMP group and CS group in repairing the liver function and liver pathology including ultrastructure,improving the perfusate acidic environment,and increasing adenosine triphosphate level (P ＜ 0.05).The oxygen consumption of NMP group and N + B group were significantly different after 2h [2 h:(24.35 ±0.64) ml/min vs.(29.33 ±0.47) ml/min;3 h:(25.33 ±0.86) ml/min vs.(30.34 ± 0.49) ml/min;4 h:(26.88 ± 1.07) ml/min vs.(31.76 ± 0.96) ml/min;P ＜ 0.05],suggesting that the liver condition in N + B group was significantly better than that in the other two groups.Conclusion Bone marrow mesenchymal stem cells could obviously repair the DCD grafts under normal temperature mechanical perfusion.
