Prevalence of CTX-M-type Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea, 2003.
- Author:
Jung Hyun LEE
1
;
Il Kwon BAE
;
Su Bong KWON
;
Seok Hoon JEONG
;
Gun Jo WOO
;
Jongwook LEE
;
Wee Gyo LEE
;
Jung Oak KANG
;
Ji Young AHN
;
Seong Geun HONG
;
Jong Hee SHIN
;
Young UH
;
Yeon Jun PARK
;
Eui Chong KIM
;
Kyungwon LEE
;
Dongeun YONG
Author Information
1. Department of Pediatrics, Kosin University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Extended-Spectrum beta-Lactamase;
CTX-M-3;
CTX-M-14;
CTX-M-15
- MeSH:
Agar;
Anti-Bacterial Agents;
beta-Lactamases;
Cefotaxime;
Diffusion;
Escherichia coli*;
Escherichia*;
Isoelectric Focusing;
Klebsiella pneumoniae*;
Klebsiella*;
Korea*;
Pneumonia;
Polymerase Chain Reaction;
Prevalence*
- From:Korean Journal of Clinical Microbiology
2004;7(2):111-118
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The aims of this study were to survey nationwide susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-Mtype extended-spectrum beta-lactamases(ESBLs). METHODS: During the period of February to July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production was determined by the double disk synergy test. Cefotaxime-resistance of the ESBL-producers was transfered to E. coli DH5alphaand E. coli Top10-F by transformation. MICs of beta-lactam antibiotics were determined by the agar dilution method. Searches for blaCTX-M genes was performed by PCR amplication; pIs of beta-lactamases were determined by isoelectric focusing. RESULTS: Among 230 isolates of E. coli and 232 isolates of K. pneumoniae, 27 (11.7%) and 79 (34.1%) were intermediate or resistant to cefotaxime, respectively. Twenty-four (10.4%) isolates of E. coli and 58 (25.0%) K. pneumoniae isolates showed positive results in the double disk synergy test. Three isolates of E. coli and 13 K. pneumoniae isolates harbored blaCTX-M-3 gene, 4 E. coli isolates harbored blaCTX-M-15 gene, and 1 E. coli and 5 K. pneumoniae isolates harbored blaCTX-M-14 gene. CONCLUSION: E. coli and K. pneumoniae isolates producing CTX-M-type ESBLs were not uncommon in Korean hospitals. It is thought that periodical surveys are necessary for inspecting the spread of CTX-M-type ESBL genes are necessary.
