Relationship between the Change Rules of Volatile Organic Compounds in Rat Muscle and Postmortem Interval
10.3969/j.issn.1004-5619.2017.02.003
- VernacularTitle:大鼠肌肉挥发性有机化合物变化规律与死亡时间的关系
- Author:
Beibei LIU
;
Zhiyuan XIA
;
Jinqi MA
;
Pu LI
;
Ping Lü
;
Haimei ZHOU
- Keywords:
forensic pathology;
volatile organic com pounds;
gas chrom atography-m ass spectrom etry;
solid phase m icroextraction;
postm ortem interval;
m uscles;
rats
- From:Journal of Forensic Medicine
2017;33(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o explore the relationship betw een the change rules of volatile organic com pounds (V O C s) in rat m uscle and postm ortem interval (PM I). Methods A total of 120 healthy rats w ere divided random ly into 12 groups (10 for each group). A fter the rats w ere sacrificed by cervical dislocation, the bodies w ere kept at (25±1)℃. R at m uscle sam ples w ere separately obtained at 12 PM I points, including 0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 d. T he V O C s in rat m uscles w ere collected, detected and ana-lyzed by headspace solid-phase m icroextraction (H S-SPM E ) coupled to gas chrom atography-m ass spec-trom eter (G C-M S ). Results In total, 15 species of V O C s w ere identified, including 9 arom atic com-pounds, 3 sulfur com pounds, 2 aliphatic acids and 1 heterocyclic com pound. T he species of V O C s in-creased w ith PM I: no species w ere detected w ithin 1 day, 3 species w ere detected on day 2, 9 on day 3, 11 on day 4, 14 from day 5 to 7, and 15 from day 8 to 10. T otal peak area of 15 species of V O C s w as significantly correlated to PM I (adjusted R2=0.15-0.96): the regression function w as y=-17.05 x2+164.36 x-246.36 (adjusted R2=0.96) from day 2 to 5, and y=2.24 x+101.13 (adjusted R2=0.97) from day 6 to 10. Conclusion T he change rules of V O C s in rat m uscle are helpful for PM I estim ation.
