Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases
10.3969/j.issn.1004-5619.2017.02.008
- VernacularTitle:硫化氢中毒案件中血液硫离子的测定
- Author:
Huosheng QIANG
;
Hang CHEN
;
Baohua SHEN
;
Min SHEN
;
Ping XIANG
- Keywords:
forensic toxicology;
poisoning;
hydrogen sulfide;
derivatization;
gas chrom atography-m ass spectrom etry
- From:
Journal of Forensic Medicine
2017;33(2):148-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o establish a gas chrom atography-m ass spectrom etry (G C-M S ) m ethod for the determ ination of sulfide ion in blood and apply it to the practical cases. Methods T he 1, 3, 5-tribro-m obenzene w as selected as an internal standard, and 0.2 m L blood sam ple w as collected and analyzed using G C-M S after α-B rom o-2, 3, 4, 5, 6-pentafluorobenzyl brom ide derivatization. Results T he m ass concentration of sulfide ion in blood had good linearity in the range of 0.2-40μg/m L w ith a lim it of detection (L O D ) of 0.05μg/m L . T he m ass concentration of sulfide ion w as less than 0.05μg/m L in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sul-fide ion w as detected in the blood sam ples of 6 victim s, and the m ass concentration range w as 1.02-3.13μg/m L . Conclusion T his study establishes a m ethod for investigation of sulfide ion in blood w hich has been applied successfully to the cases of fatal sulfide poisonings.
