Investigation on the interaction of human testis protein SPAG4L with KASH domain protein Nesprin-3
10.13602/j.cnki.jcls.2017.03.18
- VernacularTitle:人睾丸蛋白SPAG4L与KASH结构域蛋白Nesprin-3的相互作用
- Author:
Yao REN
;
Yong WU
;
Lihua HUANG
;
Xiaowei XING
- Keywords:
sperm-associated antigen 4 like;
nuclear envelop spectrin repeat proteins 3;
interaction;
LINC complex;
spermatogenesis
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(3):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interaction of human testis sperm-associated antigen 4 like(SPAG4L) protein containing Sad,UNC-84(SUN) domain with nuclear envelop spectrin repeat proteins 3 (Nesprin-3) containing Klarsicht,ANC-1 and Syne homology (KASH) domain.Methods SPAC-4L protein domains were analyzed with the bioinformatics method.After the SPAG4L plasmid was transfected into Ntera-2 cells,the subcellular localization of SPAG4L was observed.The interaction of SPAG4L with Nesprin-3 was determined by immunofluorescence,co-immunoprecipitation(co-IP) and bimolecular fluorescence complementation (BiFC) technology,respectively.Results Bioinformatics analysis results showed that there was a transmembrane structure in SPAG4L protein.Subcellular localization results demonstrated that SPAG4L protein located in the nuclear membrane and cytoplasm.Immunofluorescence,Co-IP,and BiFC results showed that there was an interaction between SPAG4L and Nesprin-3,and that a LINC(linkers of the nucleoskeleton to the cytoskeletan) complex was formed.Conclusion SPAG4L may interact with Nesprin-3 to form a LINC complex,which is important for understanding the function of SPAG4L protein in spermatogenesis.
